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Snap Chamber for Ussing System
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- Reservoir Opening: 12 mm
- Half-Chamber Volume: 1.7 mL
- Pin Circle Diameter: N/A
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| Description |
Features
WPI’s classical Ussing Chambers are well established perfusion chambers that are easy to operate, easy to control temperature, and easy to clean after use. The Ussing Chambers are machined from solid acrylic with eight entry ports for fluid lines, electrodes, or agar bridges. For easy, leak-free attachment of tubing and electrodes, all eight ports are luer type. The four ports for voltage and current electrodes are recessed to prevent formation of air bubbles in the chamber. The fluid compartments in each side of the chamber are separated by the epithelial membrane being studied. Sharp stainless steel pins on one side of the chamber hold the membrane in position and mate with holes in the opposite chamber interface.
Specific details for CMH5
The CHM5 chamber adapts the Costar Snapwell, a cell culture insert for monolayer cell culture, into WPI’s “classical” epithelial voltage clamp system. Until now, classical Ussing Chambers have not been widely used for monolayer cell culture inserts because most inserts have a very deep profile, limiting good fluid perfusion at the surface of the membrane - and limiting voltage electrodes from measuring the potential close to the surface of the membrane. CHM5 solves these problems: Perfusion fluid is introduced into the chamber at an angle so that it will flow directly to the surface of the membrane. The voltage electrode is also inserted into the chamber at an angle so as to reduce the distance between the surface of the membrane and the electrode.
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| Specifications |
Additional information not available |
| Manuals and Resources |
Additional information not available |
| References |
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| Ordering Information |
To place an order for this product, please contact the appropriate WPI office or distributor.
Customers can order direct from our China office:
WPI China
Address: Rm.20A, No.8 Dong Fang Rd, Liang Feng Building, Lu Jia Zui Financial District, Shanghai PRC
Phone:+86 21 68885517
Fax:+86 21 68885537
Code:200120
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| Accessories |
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| Application Notes |
Application Notes
No Application Notes available for this product at this time. |
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