| Description |
Precision
Maintenance of the desired temperature set-point can now be achieved through the use of a precision, shielded temperature probe coupled to a peripheral Air-Therm heating unit. Noise is a major concern in any experimental application. WPI's Air-Therm™ is electrically quiet as well as acoustically quiet). Since the Air-Therm can be placed away from the incubator, any residual electrical and vibrational interference from the heater is virtually eliminated. Accuracy of ±0.1°C can be routinely maintained at the sample site (using a fully programmable differential algorithm to automatically adjust the temperature on the Air-Therm unit). Air-Therm maintains a uniform temperature across the microscope stage (allowing for uniform heating of multiwell dishes). These tight tolerances result in minimal focal drift once sample/incubator equilibrium has been achieved.
Glass bottom dishes
For high numerical aperture (NA) live-cell imaging, cells must be plated on glass (e.g., WPI’s FluoroDish Glass Bottom Dishes, spotting 0.17 mm glass thickness) or standard cover slips. Since cover slips inherit other problems associated with sterility and accidental damage to expensive microscope condensers, WPI designed new and improved glass bottom dishes (35 mm x 10 mm or 35 mm X 22 mm) that are not cytotoxic. A special adhesive in conjunction with an advanced dish design makes the FluoroDish the only non-cytotoxic glass dish in the world. (See page 210.) This means that cells can actually be cultured for 4 to 5 days in the same dish and then carry the complete cell culture with media from the incubator directly into the (Air-Therm controlled) recording chamber. This is a comfort level that hitherto was unattainable by scientists and clinical researchers since cells will have to be transported from the culture dishes on to cover slips just prior to imagery or attaching patch electrodes.
Chamber
The most problematic aspect of any live cell imaging procedure or patch clamp approach is the thermal drift of high magnification lenses caused by temperature fluctuations in the microscope's environment. It is necessary to constantly readjust the focus during image acquisition due to this thermal-drift, introducing artifacts during quantitative microscopy due to the photo-bleaching that occurs during refocusing. The optimal solution to this problem is to use WPI’s Air-Therm temperature controller connected to a Plexiglas or acrylic chamber that encloses the entire imaging area (or at least the objectives and the stage). Using such a chamber will result in highly stable temperature control and minimal thermal drift.
Includes
2 pieces of 4.5 ft. clear, coli-reinforced heater hose included with Air-Therm |
| Specifications |
| AIR FLOW RATE | 20 to 50 CFM (0.55 to 1.4 cubic meter/M) | | CONTROL TEMP. RANGE | Ambient to 60°C | | TEMPERATURE RESOLUTION | 0.1 °C | | TEMPERATURE ACCURACY | 0.2 °C | | CONTROL MODES | (a) Auto (PID set manually, or Self-Tune), (b) Manual tune | | ANALOG OUTPUT | 0.5°C resolution | | FOR CHART RECORDER | 0-10V, 0 to 100 C (default, adjustable via controller) | | HEATING VOLUME | More than 4 cu ft (85 liter), non-circulating | | POWER (CE) | 300 W, 95-135 V or 220-240 V, 50/60 Hz | | DIMENSIONS | 61?2 x 8 x 71?2 in. (15.5 x 21 x 19 cm) | | SHIPPING WEIGHT | 10 lb (4.5 kg) |
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| Manuals and Resources |
Instruction Manual
temp-control-instructions.pdf |
| References |
T. Euler, R. H. Masland. "Light-Evoked Responses of Bipolar Cells in a Mammalian Retina." J Neurophysiol 83. 2000: 1817-1829.
X. Yin, D. A. Knecht, M.A. Lynes. "Metallothionein mediates leukocyte chemotaxis." BMC Immunology 6. 2005: 1--12.
H.E. Way, D. P. Conover, P. Mahia, M. Toraason, W. G. Lotz. "50-Hertz Magnetic Field and Calcium Transients in Jurkat Cells: Results of a Research and Public Informaiton dissemination (RAPID) Program Study." Environ Health Perspect 108. 2000: 135-140.
T. Karpova, S. L. Reck-Peterson, N. B. Elkind, M.S. Mooseker,P.J. Novick, J.A. Cooper. "Role of Actin and Myo2p I Polarized Secretion and Growth of Saccharomyces cerevisiae ." Molecular Biology of the Cell 11. 2000: 1727-1737.
R. E. Anderson, F. B. Meyer. "Is intracellular brain pH a dependent factor in NOS inhibiion during focal cerebral ischemia?." Brain Research 856. 2000: 220-236.
R.E. Anderson, F.B. Meyer. "Protection of Focal Cerebral Ischemia by Alkalinizaion f Systemic pH." Neurosurgery 51. 2002: 1256-1266.
X. Yin, D.A. Knecht, M.A. Lynes. "Meallothionein mediates leukocyte chemotaxis." BMC Immunology 6. 2005: 6--21. |
| Ordering Information |
To place an order for this product, please contact the appropriate WPI office or distributor.
Customers can order direct from our China office:
WPI China
Address: Rm.20A, No.8 Dong Fang Rd, Liang Feng Building, Lu Jia Zui Financial District, Shanghai PRC
Phone:+86 21 68885517
Fax:+86 21 68885537
Code:200120
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| Accessories |
15590 - Clear Hose for Air-Therm
300276 - Replacement Platinum Temperature Probe for Air-Therm
3491 - Extension cable, 5 feet |
| Application Notes |
Application Notes
No Application Notes available for this product at this time. |
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