| Description |
Injection volumes
WPI's microprocessor-controlled Nanoliter 2000 uses direct piston displacement. By either pushing the injection button on the control box or pressing on the optional footswitch, a calibrated volume will be smoothly injected. The process is quiet and vibration free. Capillary filling and injection speeds are 23 nL/sec and 46 nL/sec (emptying speeds are 92 nL/sec and 230 nL/sec). Maximum fluid ejection is 5 µL. Each unit comes with sufficient glass to pull at least 300 tips. Glass is 1.14 mm O.D. (nominal) and 0.5mm I.D. By setting the DIP switch, the injection volume can be changed from 2.3 to 69.0 nL in 16 steps. Up to 100 injections may be triggered per filling. Since the volume of a normal Xenopus oocyte is about 500 nL, the instrument has the capability to inject from less than 1% to over 10% of the total volume of the oocyte in one preset step increment.
Included with pump
Included: 1 vial 3.5 in. capillaries (100); 1 vial 7 in. capillaries (100); replacement "O" rings; Allen wrench; MicroFil™MF34G backfilling needle; and two sample pre-pulled micropipettes.
Smart controller
SYS-MICRO4, an optional microprocessor-based controller, can provide an "intelligent" and easy-to-use interface to up to four Nanoliter Injectors. Operating parameters are set with the membrane key-pad and LCD display. From the keypad the user can set pump to infusion or withdrawal mode, enter the volume to be infused or withdrawn, and rate of delivery, as well as synchronize the starting and stopping of a combination of Injectors. User parameters can be stored in the device's "non-volatile" memory for instant recall when the unit is powered on. An optional footswitch can be plugged into a connector on the rear of the controller for "hands free" start/stop operation. An RS-232 port on the rear of the controller can be used to connect it to a computer for use with computer control programs. |
| Specifications |
Additional information not available |
| Manuals and Resources |
Instruction Manual
NANO2000-IM-081203.pdf
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| References |
A.J. Grottick, G. Trube, W.A. Corrigal, J. Huwyler, P. Malhere, R. Wyler, G.A. Higgins. "Evidence That Nicotinic α7 Receptors are Not Involved in the Hyperlocomotor and Rewarding Effectsof Nicotine." J of Pharmacology and Experimental Therapeutics 294. 2000: 1112-1119.
I. Ronnestad, R. Perez Dominguez, M. Tanaka. "Ontogeny of digestive tract functionality in Japanese flounder, Paralichthys olvaceus studied by in vivo microinjection: pH and assimilationof free amino acids." Fish Physiology and Biochemistry 22. 2000: 225-235.
H.J. Mosson, E.H. Richards, G.C. Marris, J.P. Edwards. "The Titres of Lectins in the haemolymph of Lacanobia oleracea and the effects of parasitism by the ectoparasitoid wasp Eulophus pennicornis." Physiological Entomology 25. 2000: 296-302.
S. Supplisson, D. Chesnoy-Machais. "Glycine Receptor β Subunits Play a Critical Role in Potentiationof Glycine Responses by ICS-205,903." Molecular Pharmacoloy 58. 2000: 763-770.
V.R. Zhelyaskov, S.Y. Liu, M.P Broderick. "Analysis of nanoliter samples of electrolytes using a flow-through microfluorometer." Kidney International 57. 2000: 1764-1769.
W.J. Welch, J.Blau, H. Xie, t. Chabrashvili, C.S. Wilcox. "AT1 receptor antagonist combats oxidative stress and restores nitric oxide signalin in the SHR." Kidney International 59. 2001: 1257-1263.
H.L. Ji, C.M. Fuller, D.J. Benos. "Peptide Inhibition of Constitutively Activated Epithelial Na+ Channels Expressed in Xenopus Oocytes." J of Biological Chemistry 274. 1999: 37693-37704.
VS Mujumdar, LM Smiley, SC Tyagi. "Activation of matrix metalloproteinase dilates and decreases catensile strength." Can J Physiol Pharmacol 77. 1999: 950-957.
S. Nishlike, W.O. Guldin, J. Baurle. "Corticofungal connections between the cerebral cortex and the vestibular nuclei in the rat." J of Comparative Neurology 420. 2000: 363-372.
T. Nakada, K. Zandi-Nejad, Y. Kurita, H. Kudo, V. Broumand, c. Y. Kwon, A. Mercado, D.B. Mount, S. Hirose. "Roles of SIc13a1 and Slc26a1 sulfate transporters of eel kidney in sulfate homeostasis and osmoregulation in freshwater." Am J Physiol Regul Integr Comp Physiol 289. 2005: R575-R585.
T. Nakada, K. Zandi-Nejad, Y. Kurita, H. Kudo, V. Broumand, c. Y. Kwon, A. Mercado, D.B. Mount, S. Hirose. "Roles of SIc13a1 and Slc26a1 sulfate transporters of eel kidney in sulfate homeostasis and osmoregulation in freshwater." Am J Physiol Regul Integr Comp Physiol 289. 2005: R575-R585.
A.J. Grottick, G. Trube, W.A. Corrigal, J. Huwyler, P. Malhere, R. Wyler, G.A. Higgins. "Evidence That Nicotinic α7 Receptors are Not Involved in the Hyperlocomotor and Rewarding Effectsof Nicotine." J of Pharmacology and Experimental Therapeutics 294. 2000: 1112-1119.
S.J. Noh, M.J. Kim, S. Shim, J.K. Han. "Different signaling pathway between sphingosine-1-phosphate and lysophosphatidic acid in Xenopusoocytes: Functional coupling of the sphingosine-1-1Phosphate receptor to PLC-x? in Xeonopus oocytes." J of Cellular Physiology 17. 1998: 412-423.
Wilcox & Welch. "Interaction between nitric oxide and oxygen radicals in regulation of tubuloglomerular feedback." Acta Physiologica Scandinavica 168. 2000: 119-124.
W.J. Wlch, A. Tojo, C.S. Wilcox. "Roles of NO and oxygen radicals in tubuloglomerular feedback in SHR." Am J Physiol Renal Physiol 278. 2000: F769-F776. |
| Ordering Information |
To place an order for this product, please contact the appropriate WPI office or distributor.
Customers can order direct from our China office:
WPI China
Address: Rm.20A, No.8 Dong Fang Rd, Liang Feng Building, Lu Jia Zui Financial District, Shanghai PRC
Phone:+86 21 68885517
Fax:+86 21 68885537
Code:200120
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| Accessories |
13142 - Optional Foot Switch for DMF1000 Microforge
15867 - Footswitch for SYS-Micro4 Controller
4878 - Glass Capillaries for Nanoliter 2000
4879 - Glass Capillaries for Nanoliter 2001
TIP10XV119 - Micropipette Tips for Nanoliter 2000
300033 - Nanoliter 2000 adapter for SYS-Micro4
5340 - Spare Parts Kit for Nanoliter 2000
500778 - Universal Adapter for Nanoliter Injector
502859 - Flared O-ring Kit for Nanoliter 2000
500299 - Pistons for Nanoliter 2000
503066 - Flared Glass for Nanoliter 2000
SYS-MICRO4 - Four channel micro controller |
| Application Notes |
Application Notes
No Application Notes available for this product at this time. |
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